A collection of tools to analyze 2-photon intravital microscopy videos of immune cells

Cell action recognition (track-based)

This tool detects and counts the actions of neutrophils as described in Pizzagalli et al. Frontiers in Immunology (2019)

  • Program for Windows (coming soon)
  • MATLAB script (download) - Requires Matlab to be executed
  • Imaris XTension (coming soon)

Semi-supervised Colocalization

This tool creates an additional imaging channel, specific for the cells of interest.

The user can draw a few lines on the cells of interest and other lines on the background.
The program will automatically separate them into 2 classes.

Useful when cells appear in more than one acquisition channel.

  • Imaris XTension (download) (expected to be available by December 2019)

NOTE: THIS IS A PRELIMINARY VERSION. The full software that facilitates colocalization and cell tracking will come soon.




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